Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (cat. 11-0038) and Mouse IgG1 κ Isotype Control eFluor® 660 (cat. 50-4714) (left) or Anti-Human Vα24Jα18 TCR eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human V alpha 24 J alpha 18 TCR eFluor® 660 (Alexa Fluor® 647 replacement) Catalog Number: 50-5806 Concentration: 5 µL (0.25 µg)/test Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material. Clone: 6B11 Host/Isotype: Mouse IgG1, k
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Size
Price
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50-5806-41
25 tests
50-5806-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 6B11 monoclonal antibody reacts with an epitope of the CDR3 formed by the germ-line configuration of the Vα24 and Jα18 of the TCR α locus, which results in the expression of an invariant T-cell receptor (TCR) expressed by invariant NK T cells (iNKT). This TCR is homologous to the murine Vα14Jα18. This TCR α chain pairs with a restricted set of rearranged TCR β chains, with Vβ11 being the most prominent in humans. NK T cells are restricted by the CD1d molecule and are activated by a CD1d-presented glycolipid ligand, which results in the rapid production of IL-4, IL-13 and IFNγ. Although iNKT cells have NK-like cytolytic activity, they are considered regulators of immune responses because they rapidly produce large amounts of both Th1 and Th2 cytokines in autoimmune disease, infectious disease, and cancer. The number of iNKT cells present in peripheral blood of normal humans shows considerable variability ranging from 0.01-1% of total peripheral T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 6B11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 6B11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
References
Montoya CJ, Pollard D, Martinson J, Kumari K, Wasserfall C, Mulder CB, Rugeles MT, Atkinson MA, Landay AL, Wilson SB.Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11.Immunology. 2007 Sep;122(1):1-14. (6B11, mAb characterization, PubMed)
Thomas SY, Hou R, Boyson JE, Means TK, Hess C, Olson DP, Strominger JL, Brenner MB, Gumperz JE, Wilson SB, Luster AD.CD1d-restricted NKT cells express a chemokine receptor profile indicative of Th1-type inflammatory homing cells.J Immunol. 2003 Sep 1;171(5):2571-80. (6B11, FC, PubMed)
Illés Z, Kondo T, Newcombe J, Oka N, Tabira T, Yamamura T.Differential expression of NK T cell V alpha 24J alpha Q invariant TCR chain in the lesions of multiple sclerosis and chronic inflammatory demyelinating polyneuropathy.J Immunol. 2000 Apr 15;164(8):4375-81.
