Staining of normal human peripheral blood cells with Anti-Human CD3 APC (cat. 17-0038) and Mouse IgG2b κ Isotype Control eFluor® 450 (cat. 48-4732) (left) or Anti-Human V beta 8 TCR eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human V beta 8 TCR eFluor® 450 Catalog Number: 48-9890 Concentration: 5 µl (0.25 µg)/test Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material. Clone: JR-2 Host/Isotype: Mouse IgG2b, k
Product Options
Cat. No.
Size
Price
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48-9890-41
25 tests
48-9890-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
eFluor® products are not offered for sale or use in certain regions. Please contact an eBioscience representative for product availability.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This JR-2 monoclonal antibody reacts with the human T cell receptor (TCR) Vβ 8 allele. Composed of an α and β chain, TCR specificity is typically determined by Vα, Jα, Vβ, Dβ, and Jβ gene rearrangement. Vß expression in humans has been examined in studies on the effects of superantigens, inflammation, autoimmune disease, and HIV infection. More recently, assessment of TCR Vβ expression has been used to phenotype T cell clonality in CD3+/TCRαβ+ large granular lymphocyte leukemias. A member of the Ig superfamily, this receptor is expressed on a subset of peripheral blood T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This JR-2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This JR-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 456 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
References
Lima M, Almeida J, Santos AH, dos Anjos Teixeira M, Alguero MC, Queirós ML, Balanzategui A, Justiça B, Gonzalez M, San Miguel JF, Orfao A. Immunophenotypic analysis of the TCR-Vbeta repertoire in 98 persistent expansions of CD3(+)/TCR-alphabeta(+) large granular lymphocytes: utility in assessing clonality and insights into the pathogenesis of the disease. Am J Pathol. 2001 Nov;159(5):1861-8.
Stuart PM, Munn RK, DeMoll E, Woodward JG. Characterization of human T-cell responses to Yersinia enterocolitica superantigen. Hum Immunol. 1995 Aug;43(4):269-75. (JR-2, FC)
Choi YW, Kotzin B, Lafferty J, White J, Pigeon M, Kubo R, Kappler J, Marrack P. A method for production of antibodies to human T-cell receptor beta-chain variable regions. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8357-61.
