Staining of bEnd.3 cell line with 1 µg of Rat IgG1 κ Isotype Control eFluor® 450 (cat. 48-4301) (blue histogram) or 1 µg of Anti-Mouse CD144 (VE-Cadherin) eFluor® 450 (purple histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD144 (VE-Cadherin) eFluor® 450 (Pacific Blue® replacement) Catalog Number: 48-1441 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBioBV13 (BV13) Host/Isotype: Rat IgG1
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Cat. No.
Size
Price
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48-1441-80
25 ug
48-1441-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
eFluor® products are not offered for sale or use in certain regions. Please contact an eBioscience representative for product availability.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 2 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluorTM 450 is a replacement for Pacific Blue®. eFluorTM 450 emits at 456 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
References
Corada M, Mariotti M, Thurston G, Smith K, Kunkel R, Brockhaus M, Lampugnani MG, Martin-Padura I, Stoppacciaro A, Ruco L, McDonald DM, Ward PA, Dejana E.
Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo.
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9815-20. (BV13, FA, IHC, PubMed)
Liao F, Li Y, O'Connor W, Zanetta L, Bassi R, Santiago A, Overholser J, Hooper A, Mignatti P, Dejana E, Hicklin DJ, Bohlen P.
Monoclonal antibody to vascular endothelial-cadherin is a potent inhibitor of angiogenesis, tumor growth, and metastasis.
Cancer Res. 2000 Dec 15;60(24):6805-10. (BV13, FA, PubMed)
Crosby CV, Fleming PA, Argraves WS, Corada M, Zanetta L, Dejana E, Drake CJ.
VE-cadherin is not required for the formation of nascent blood vessels but acts to prevent their disassembly.
Blood. 2005 Apr 1;105(7):2771-6. Epub 2004 Dec 16. (BV13, FA, PubMed)
