Staining of C57Bl/6 splenocytes with 0.5 μg of eFluor™ 450 Rat IgG2a Isotype Control (cat. 48-4321) (blue histogram) or 0.5 μg of eFluor™ 450 anti-mouse ICAM-2 (CD102)(purple histogram). Total viable cells were used for analysis.
Contents: eFluor® 450 anti-mouse ICAM-2 (CD102) Catalog Number: 48-1021 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: 3C4 (mIC2/4) Host/Isotype: Rat IgG2a, κ
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Cat. No.
Size
Price
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48-1021-80
25 ug
48-1021-82
100 ug
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eFluor products are not offered for sale or use in certain regions. Please contact an eBioscience representative for more information on whether our products are available for purchase in your area.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The monoclonal antibody 3C4 (mIC2/4) recognizes mouse CD102, also known as intercellular adhesion molecule-2 (ICAM-2). CD102, a member of the Immunoglobulin superfamily of receptors with 2 extracellular Ig domains, binds LFA (CD11a/CD18) and Mac-1 (CD11b/CD18), thereby mediating cellular interactions to lymphocytes. CD102 plays a role in NK cytotoxicity and migration, and lymphocyte recirculation. Expression of CD102 is found on most lymphocytes, monocytes and platelets.
The 3C4 (mIC2/4) antibody blocks interaction of lymphocytes with antigen presenting cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 3C4 (mIC2/4) antibody has been reported for use in flow cytometric analysis.
eFluorTM 450 is a replacement for Pacific Blue®. eFluorTM 450 emits at 456 nm and is excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This 3C4 (mIC2/4) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Gerwin N, Gonzalo JA, Lloyd C, Coyle AJ, Reiss Y, Banu N, Wang B, Xu H, Avraham H, Engelhardt B, Springer TA, Gutierrez-Ramos JC. Prolonged eosinophil accumulation in allergic lung interstitium of ICAM-2 deficient mice results in extended hyperresponsiveness. Immunity. 1999 Jan;10(1):9-19. (3C4, FC, IHC/IF, PubMed)
Xu H, Bickford JK, Luther E, Carpenito C, Takei F, Springer TA. Characterization of murine intercellular adhesion molecule-2. J Immunol. 1996 Jun 15;156(12):4909-14. (3C4, mAb characterization, IP, FA, PubMed)
