Staining of C57BL/6 splenocytes with 0.5 µg of Rat IgG2a κ Isotype Control eFluor® 450 (cat. 48-4321) (blue histogram) or 0.5 µg of Anti-Mouse CD102 (ICAM-2) eFluor® 450 (purple histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD102 (ICAM-2) eFluor® 450 (Pacific Blue® replacement) Catalog Number: 48-1021 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 3C4 (mIC2/4) Host/Isotype: Rat IgG2a, κ
Product Options
Cat. No.
Size
Price
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48-1021-80
25 ug
48-1021-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
eFluor® products are not offered for sale or use in certain regions. Please contact an eBioscience representative for product availability.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The monoclonal antibody 3C4 (mIC2/4) recognizes mouse CD102, also known as intercellular adhesion molecule-2 (ICAM-2). CD102, a member of the Immunoglobulin superfamily of receptors with 2 extracellular Ig domains, binds LFA (CD11a/CD18) and Mac-1 (CD11b/CD18), thereby mediating cellular interactions to lymphocytes. CD102 plays a role in NK cytotoxicity and migration, and lymphocyte recirculation. Expression of CD102 is found on most lymphocytes, monocytes and platelets.
The 3C4 (mIC2/4) antibody blocks interaction of lymphocytes with antigen presenting cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 3C4 (mIC2/4) antibody has been reported for use in flow cytometric analysis.
eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 456 nm and is excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This 3C4 (mIC2/4) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluorTM 450 is a replacement for Pacific Blue®. eFluorTM 450 emits at 456 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
References
Gerwin N, Gonzalo JA, Lloyd C, Coyle AJ, Reiss Y, Banu N, Wang B, Xu H, Avraham H, Engelhardt B, Springer TA, Gutierrez-Ramos JC. Prolonged eosinophil accumulation in allergic lung interstitium of ICAM-2 deficient mice results in extended hyperresponsiveness. Immunity. 1999 Jan;10(1):9-19. (3C4, FC, IHC/IF, PubMed)
Xu H, Bickford JK, Luther E, Carpenito C, Takei F, Springer TA. Characterization of murine intercellular adhesion molecule-2. J Immunol. 1996 Jun 15;156(12):4909-14. (3C4, mAb characterization, IP, FA, PubMed)
