Product Information

Contents: Allophycocyanin-eFluor^TM 780 anti-mouse CD117 (c-Kit, cKit)
Catalog Number: 47-1171
Sizes: 25 ug, 100 ug
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 2B8
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 bone marrow cells with PE anti-mouse Sca-1 (D7) (cat. 12-5981) and staining buffer (autofluorescence) (left) or 0.06 μg of APC-eFluorTM 780 anti-mouse CD117 (2B8) (right). Gated Lineage Low cells (stained with Pacific Blue Lineage cocktail (cat. 88-7773)) were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1171	Affinity Purified anti-mouse CD117 (c-Kit, cKit)	N/A	N/A	FC IP
13-1171	Biotin anti-mouse CD117 (c-Kit, cKit)	N/A	N/A	FC
11-1171	FITC anti-mouse CD117 (c-Kit, cKit)	488	518	FC
12-1171	PE anti-mouse CD117 (c-Kit, cKit)	488	575	FC
15-1171	PE-Cy5 anti-mouse CD117 (c-Kit, cKit)	488	670	FC
17-1171	APC anti-mouse CD117 (c-Kit, cKit)	633	660	FC
19-1171	Cy5 anti-mouse CD117 (c-Kit, cKit)	633	670	FC
25-1171	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD117 (c-Kit, cKit)	488	760	FC
35-1171	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD117 (c-Kit, cKit)	488	690	FC
47-1171	Allophycocyanin-eFluor^TM 780 anti-mouse CD117 (c-Kit, cKit) (APC-Alexa Fluor® 750 replacement)	633	780	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2B8 antibody has been reported for use in flow cytometric analysis.

APC-eFluor^TM 780 is a replacement for APC-Alexa Fluor® 750. APC-eFluor^TM 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Caution should be used when fixing samples stained with APC-eFluor^TM 780 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ikuta K, Weissman IL. 1992. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Proc Natl Acad Sci USA. 89(4): 1502-6.

Related Products

Cat. 47-4031 Allophycocyanin-eFluor™ 780 Rat IgG2b Isotype Control (APC-Alexa Fluor® 750 replacement)