Staining of C57Bl/6 splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.06 μg of APC-eFluorTM 780 Rat IgG2a Isotype Control (cat. 47-4321) (left) or 0.06 μg of APC-eFluorTM 780 anti-mouse CD90.2 (53-2.1) (right). Total viable cells were used for analysis.
Contents: Allophycocyanin-eFluorŪ 780 anti-mouse CD90.2 (Thy-1.2, Thy1.2) Catalog Number: 47-0902 Concentration: 0.2 mg/mL Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly. Clone: 53-2.1 Host/Isotype: Rat IgG2a, κ
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Size
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47-0902-80
25 ug
47-0902-82
100 ug
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Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 53-2.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor® 780 is a replacement for APC-Alexa FluorŪ 750. APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Caution should be used when fixing samples stained with APC-eFluor® 780 as this may lead to degradation of the fluorochrome and loss of staining.
References
Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.
