Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (cat. 11-0452) and 0.5 µg of Rat IgG2b κ Isotype Control APC-eFluor® 780 (cat. 47-4031) (left) or 0.5 µg of Anti-Mouse CD3 APC-eFluor® 780 (right). Total viable cells were used for analysis.
Contents: Anti-Mouse CD3 APC-eFluor® 780 Catalog Number: 47-0032 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly. Clone: 17A2 Host/Isotype: Rat IgG2b, κ
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Cat. No.
Size
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47-0032-80
25 ug
47-0032-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
eFluor® products are not offered for sale or use in certain regions. Please contact an eBioscience representative for product availability.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits γ, δ and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 17A2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
APC-eFluor® 780 is a replacement for APC-Alexa Fluor® 750. APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Caution should be used when fixing samples stained with APC-eFluor® 780 as this may lead to degradation of the fluorochrome and loss of staining.
References
Miescher, G. C., M. Schreyer, et al. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989: 23(2): 113-8.
Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation.
J Immunol. 2002 Mar 15;168(6):3088-98.(17A2, IH/F, PubMed)
