Also known as: Integrin Beta 1, ITGB1, Fibronectin Receptor Beta, VLA-Beta
RUO: For Research Use Only
Staining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (cat. 17-0452) and 0.5 μg of Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (cat. 46-4888) (left) or 0.5 μg of Anti-Mouse/Rat CD29 PerCP-eFluor® 710 (right). Cells in the small/lymphocyte scatter population were used for analysis.
Contents: Anti-Mouse/Rat CD29 PerCP-eFluor® 710 Catalog Number: 46-0291 Concentration: 0.2 mg/mL Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material. Clone: eBioHMb1-1 (HMb1-1) Host/Isotype: Armenian Hamster IgG
Product Options
Cat. No.
Size
Price
Add Qty to Cart
46-0291-80
25 ug
46-0291-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin β1), a 110-120 kDa member of the β integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the α integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These αβ integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Ridger VC, Wagner BE, Wallace WA, Hellewell PG. Differential effects of CD18, CD29, and CD49 integrin subunit inhibition on neutrophil migration in pulmonary inflammation. J Immunol. 2001 Mar 1;166(5):3484-90. (HMβ1-1, FA, PubMed)
Noto K, Kato K, Okumura K, Yagita H. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol. 1995 May;7(5):835-42. (HMβ1-1, mAb development, IP, FA, PubMed)
