Product Information

Contents: PerCP-Cy5.5 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)
Catalog Number: 45-7349
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MAb11
Isotype: Mouse IgG1, κ

Intracellular staining of unstimulated (left) and 5 hour PMA and Ionomycin-stimulated (in the presence of brefeldinA) normal human PBMCs (right) with APC anti-human CD4 (RPA-T4) (cat. 17-0049) and PerCP-Cy5.5 anti-human TNFa (MAb11). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7349	FITC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	518	IC Flow
12-7349	PE anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	575	IC Flow
13-7349	Biotin anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	ELISA det ELISPOT det
14-7349	Affinity Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IC Flow
17-7349	APC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	660	IC Flow
25-7349	Phycoerythrin-Cy7 (PE-Cy7) anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	760	IC Flow
45-7349	PerCP-Cy5.5 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	690	IC Flow
51-7349	Alexa Fluor® 647 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	668	IC Flow
53-7349	Alexa Fluor® 488 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	519	IC Flow
57-7349	Pacific Blue® anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	402	421	IC Flow

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-α ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MAb11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 20 μl (0.06 μg) per million cells in a 100 μl total staining volume.

References

Danis, V. A., G. M. Franic, et al. (1991). "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes." Clin Exp Immunol 85(1): 143-50.
Rathjen, D. A., K. Cowan, et al. (1991). "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." Mol Immunol 28(1-2): 79-86.

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Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control