Contents: PerCP-Cy5.5 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)
Catalog Number: 45-7349
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MAb11
Isotype: Mouse IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
May not be available for sale in all countries (see below).
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Intracellular staining of unstimulated (left) and 5 hour PMA and Ionomycin-stimulated normal human PBMCs (right) with APC anti-mouse CD4 (GK1.5) (cat. 17-0041) and PerCP-Cy5.5 anti-human TNFa (MAb11). Cells in the monocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7349 |
FITC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
518 |
IC Flow
|
| 12-7349 |
PE anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
575 |
IC Flow
|
| 13-7349 |
Biotin anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
N/A |
N/A |
ELISA det
ELISPOT det
|
| 14-7349 |
Affinity Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
N/A |
N/A |
IC Flow
|
| 17-7349 |
APC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
633 |
660 |
IC Flow
|
| 25-7349 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
760 |
IC Flow
|
| 45-7349 |
PerCP-Cy5.5 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
690 |
FC
|
| 51-7349 |
Alexa Fluor® 647 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
633 |
668 |
IC Flow
|
| 53-7349 |
Alexa Fluor® 488 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
519 |
IC Flow
|
| 57-7349 |
Pacific Blue® anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
402 |
421 |
IC Flow
|
PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-α ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4
+ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This MAb11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 20 μl (0.06 μg) per million cells in a 100 μl total staining volume.
References
Danis, V. A., G. M. Franic, et al. (1991). "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes."
Clin Exp Immunol 85(1): 143-50.
Rathjen, D. A., K. Cowan, et al. (1991). "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors."
Mol Immunol 28(1-2): 79-86.
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