Product Information

Contents: PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g)
Catalog Number: 45-7319
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4S.B3
Isotype: Mouse IgG1, κ

Intracellular staining of 5-hour PMA/Ionomycin stimulated normal human peripheral blood mononuclear cells in the presence of monensin with APC anti-human CD4 (RPA-T4) (cat. 17-0049) and 0.06 μg PerCP-Cy5.5 mouse IgG1 isotype control (cat.45-4714) (left) or PerCP-Cy5.5 anti-human IFNg (4S.B3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7319	FITC anti-human IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7319	PE anti-human IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
13-7319	Biotin anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA det ELISPOT
14-7319	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	IC Flow
17-7319	APC anti-human IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
25-7319	Phycoerythrin-Cy7 (PE-Cy7) anti-human IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
45-7319	PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow
51-7319	Alexa Fluor® 647 anti-human IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7319	Alexa Fluor® 488 anti-human IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7319	Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 4S.B3 monoclonal antibody reacts with interferon-γ (IFN-γ). The 4S.B3 antibody is a neutralizing antibody. Human IFN-γ is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This 4S.B3 antibody has been pre-titrated and tested by intracellular staining of stimulated normal human peripheral blood cells. This can be used at 20 μl (0.06 μg) per million cells in a 100 μl total staining volume.

References

Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." J Interferon Res 4(4): 619-25.

Related Products

Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control
Cat. 45-7311 PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)