Contents: PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g)
Catalog Number: 45-7319
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4S.B3
Isotype: Mouse IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
May not be available for sale in all countries (see below).
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Intracellular staining of 5-hour PMA/Ionomycin stimulated normal human peripheral blood mononuclear cells in the presence of monensin with APC anti-human CD4 (RPA-T4) (cat. 17-0049) and 0.06 μg PerCP-Cy5.5 mouse IgG1 isotype control (cat.45-4714) (left) or PerCP-Cy5.5 anti-human IFNg (4S.B3) (right). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7319 |
FITC anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
518 |
IC Flow
|
| 12-7319 |
PE anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
575 |
IC Flow
|
| 13-7319 |
Biotin anti-human IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
ELISA det
ELISPOT
|
| 14-7319 |
Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
IC Flow
|
| 17-7319 |
APC anti-human IFNg (Interferon-gamma, IFN-g) |
633 |
660 |
IC Flow
|
| 25-7319 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
760 |
IC Flow
|
| 45-7319 |
PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
690 |
IC Flow
|
| 51-7319 |
Alexa Fluor® 647 anti-human IFNg (Interferon-gamma, IFN-g) |
633 |
668 |
IC Flow
|
| 53-7319 |
Alexa Fluor® 488 anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
519 |
IC Flow
|
| 57-7319 |
Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g) |
402 |
421 |
IC Flow
|
PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 4S.B3 monoclonal antibody reacts with interferon-γ (IFN-γ). The 4S.B3 antibody is a neutralizing antibody. Human IFN-γ is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This 4S.B3 antibody has been pre-titrated and tested by intracellular staining of stimulated normal human peripheral blood cells. This can be used at 20 μl (0.06 μg) per million cells in a 100 μl total staining volume.
References
Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon."
J Interferon Res 4(4): 619-25.
Related Products
Cat.
45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control
Cat.
45-7311 PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
