Product Information

Contents: PerCP-Cy5.5 anti-human IL-17A (Interleukin-17A)
Catalog Number: 45-7179
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio64DEC17
Isotype: Mouse IgG1, κ

Intracellular staining of human PBMC's stimulated with PMA/Ionomycin for 5 hours in the presence of monensin with FITC anti-human CD3 (OKT3) (cat. 11-0039) and 0.125 μg of PerCP-Cy5.5 Mouse IgG1,K Iso Cntrl (cat. 45-4714) (left) or PerCP-Cy5.5 anti-human IL-17A (eBio64DEC17) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7179	Affinity Purified anti-human IL-17A (Interleukin-17A)	N/A	N/A	ELISA IHC(Paraffin)
13-7179	Biotin anti-human IL-17A (Interleukin-17A, IL17A)	N/A	N/A	ELISA det ELISPOT det
11-7179	Fluorescein isothiocyanate (FITC) anti-human IL-17A (Interleukin-17A)	488	518	IC Flow
12-7179	Phycoerythrin (PE) anti-human IL-17A (Interleukin-17A, IL17A)	488	575	IC Flow
17-7179	Allophycocyanin (APC) anti-human IL-17A (Interleukin-17A)	633	660	IC Flow
51-7179	Alexa Fluor® 647 anti-human IL-17A (Interleukin-17A, IL17A)	633	668	IC Flow
53-7179	Alexa Fluor® 488 anti-human IL-17A (Interleukin-17A, IL17A)	488	519	IC Flow
45-7179	PerCP-Cy5.5 anti-human IL-17A (Interleukin-17A) (PE-Cy5.5 replacement)	488	690	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies. Click here for link to data 51-7179.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio64DEC17 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBio64DEC17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Acosta-Rodriguez EV, Napolitani G, et al. 2007. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat Immunol. 8(9):942-9. (FC, PubMed)

Chen Z, Tato CM, Muul L, Laurence A, O'Shea JJ. Distinct regulation of interleukin-17 in human T helper lymphocytes. Arthritis Rheum. 2007 Sep;56(9):2936-46. (ebio64Dec17, FC PubMed)

Hirata T, Osuga Y, et al. 2008. Interleukin (IL)-17A Stimulates IL-8 Secretion, Cyclooxygensase-2 Expression, and Cell Proliferation of Endometriotic Stromal Cells. Endocrinology 149(3):1260-7. (eBio64DEC17, IHC paraffin, PubMed)

Related Products

Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control
Cat. 45-7177 PerCP-Cy5.5 anti-mouse IL-17A (Interleukin-17A) (clone eBio17B7)