Product Information
Contents: PerCP-Cy5.5 anti-mouse IL-2 (Interleukin-2)
Catalog Number: 45-7021
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: JES6-5H4
Isotype: Rat IgG2b, κ
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Staining of Mouse splenocytes unstimulated (left) and stimulated with PMA and ionomycin in the presence of Brefeldin A overnight (right). The cells were surface stained with APC anti-mouse CD4 (GK1.5) and intracellularly stained with 0.06 μg PerCP-Cy5.5 anti-mouse IL-2 (JES6-5H4).
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-7021 |
Affinity Purified anti-mouse IL-2 (Interleukin-2, IL2) |
N/A |
N/A |
IC Flow
IP
|
| 16-7021 |
Functional Grade* Purified anti-mouse IL-2 (Interleukin-2, IL2) |
N/A |
N/A |
IC Flow
|
| 13-7021 |
Biotin anti-mouse IL-2 (Interleukin-2, IL2) |
N/A |
N/A |
ELISA det
ELISPOT det
IP
|
| 11-7021 |
FITC anti-mouse IL-2 (Interleukin-2, IL2) |
488 |
518 |
IC Flow
|
| 12-7021 |
PE anti-mouse IL-2 (Interleukin-2, IL2) |
488 |
575 |
IC Flow
|
| 17-7021 |
APC anti-mouse IL-2 (Interleukin-2, IL2) |
633 |
660 |
IC Flow
|
| 25-7021 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IL-2 (Interleukin-2) |
488 |
760 |
IC Flow
|
| 36-7021 |
In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IL-2 (Interleukin-2, IL2) |
N/A |
N/A |
IVCCA
|
| 51-7021 |
Alexa Fluor® 647 anti-mouse IL-2 (Interleukin-2, IL2) |
633 |
668 |
IC Flow
|
| 53-7021 |
Alexa Fluor® 488 anti-mouse IL-2 (Interleukin-2, IL2) |
488 |
519 |
IC Flow
|
| 57-7021 |
Pacific Blue® anti-mouse IL-2 (Interleukin-2, IL2) |
402 |
421 |
IC Flow
|
| 45-7021 |
PerCP-Cy5.5 anti-mouse IL-2 (Interleukin-2) (PE-Cy5.5 replacement) |
488 |
690 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This JES6-5H4 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Abrams, J. S., M. G. Roncarolo, et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 127: 5-24.
Sander, B., I. Hoiden, et al. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Meth. 166(2): 201-14.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.
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