Product Information

Contents: PerCP-Cy5.5 anti-mouse/human ZAP-70 (ZAP70)
Catalog Number: 45-6695
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 1E7.2
Isotype: Mouse IgG1, κ

Intracellular staining of permeabilized C57Bl/6 thymus with 0.5 μg of PerCP-Cy5.5 Mouse IgG1,K Iso Cntrl (cat. 45-4714) (open histogram) or 2.0 μg of PerCP-Cy5.5 anti-mouse/human ZAP-70 (1E7.2) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-6695	FITC anti-mouse/human ZAP-70 (ZAP70)	488	518	IC Flow
12-6695	PE anti-mouse/human ZAP-70 (ZAP70)	488	575	IC Flow
13-6695	Biotin anti-mouse/human ZAP-70 (ZAP70)	N/A	N/A	WB
14-6695	Affinity Purified anti-mouse/human ZAP-70 (ZAP70)	N/A	N/A	IC Flow IP WB
35-6695	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse/human ZAP-70 (ZAP70)	488	690	IC Flow
45-6695	PerCP-Cy5.5 anti-mouse/human ZAP-70 (ZAP70)	488	690	IC Flow

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 1E7.2 antibody reacts with human and mouse ZAP-70, the TCRζ-associated protein-70. ZAP-70 is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. It is expressed in T and NK cells and is required for TCR signaling and development. ZAP-70 interacts with the TCR complex by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Following activation, ZAP-70 is phosphorylated on several tyrosine residues by two mechanisms; an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck1-3. Tyrosine phosphorylation of ZAP-70 correlates to its increased kinase activity and triggers downstream signaling events. Mutations in ZAP-70 have been shown to result in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. 1E7.2 was generated against a KLH-peptide sequence corresponding to the human ZAP-70 amino acid residues 282-307. While ZAP-70 is normally expressed in T and NK cells, several recent studies have also shown high correlation of ZAP-70 positive expression with mutated IgVH expression in B-chronic lymphocytic leukemia (CCL). In conclusion, the expression of ZAP-70, which can be measured by intracellular flow cytometry, may serve as a prognostic marker for B-CLL.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 1E7.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This 1E7.2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 4 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Qian D, Lev S, van Oers NS, Dikic I, Schlessinger J, Weiss A. 1997. Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling. J Exp Med. 185(7):1253-9.
Qian D, Mollenauer MN, Weiss A. 1996. Dominant-negative zeta-associated protein 70 inhibits T cell antigen receptor signaling. J Exp Med. 183(2):611-20.
Orchard JA, Ibbotson RE, Davis Z, Wiestner A, Rosenwald A, Thomas PW, Hamblin TJ, Staudt LM, Oscier DG. 2004. ZAP-70 expression and prognosis in chronic lymphocytic leukaemia. Lancet. Jan 10;363(9403):105-11.
Chen L, Widhopf G, Huynh L, Rassenti L, Rai KR, Weiss A, Kipps TJ. 2002. Expression of ZAP-70 is associated with increased B-cell receptor signaling in chronic lymphocytic leukemia. Blood. Dec 15;100(13):4609-14.

Related Products

Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control