Contents: PerCP-Cy5.5 anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)
Catalog Number: 45-5941
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: PK136
Isotype: Mouse IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
May not be available for sale in all countries (see below).
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Staining of C57Bl/6 splenocytes with APC anti-mouse pan-NK (DX5) (cat. 17-5971) and 0.25 μg of PerCP-Cy5.5 Mouse IgG2a, K Iso Cntrl (cat. 45-4724) (left) or 0.25 μg of PerCP-Cy5.5 anti-mouse NK-1.1 (PK136) (right). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5941 |
FITC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
518 |
FC
|
| 12-5941 |
PE anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
575 |
FC
|
| 13-5941 |
Biotin anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
N/A |
N/A |
FC
|
| 14-5941 |
Affinity Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
N/A |
N/A |
FC
IP
|
| 16-5941 |
Functional Grade* Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
N/A |
N/A |
FC
|
| 17-5941 |
APC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
633 |
660 |
FC
|
| 25-5941 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
760 |
FC
|
| 45-5941 |
PerCP-Cy5.5 anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
690 |
FC
|
| 57-5941 |
Pacific Blue® anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No.
14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5
+PK136
- and DX5
-PK136
+ cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This PK136 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This PK136 antibody has been tested by flow cytometric analysis of C57/BL6 mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Koo, G. C. and J. R. Peppard (1984). "Establishment of monoclonal anti-Nk-1.1 antibody."
Hybridoma 3(3): 301-3.
Kitaichi N, Kotake S, Morohashi T, Onoe K, Ohno S, Taylor AW. Diminution of experimental autoimmune uveoretinitis (EAU) in mice depleted of NK cells.
J Leukoc Biol. 2002 Dec;72(6):1117-21. (
PK136, in vivo depletion,
PubMed)
Related Products
Cat.
45-4724 PerCP-Cy5.5 Mouse IgG2a, K Isotype Control
Mouse Cellular Antigens Chart