Contents: PerCP-Cy5.5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)
Catalog Number: 45-5921
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: TER-119
Isotype: Rat IgG2b, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
May not be available for sale in all countries (see below).
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Staining of C57Bl/6 bone marrow cells with APC anti-mouse CD45 (30-F11) (cat. 17-0451) and 0.25 μg of PerCP-Cy5.5 Rat IgG2b Isotype Control (cat. 45-4031) (left) or 0.25 μg of PerCP-Cy5.5 anti-mouse Erythroid cells (TER-119) (right). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5921 |
FITC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
518 |
FC
|
| 12-5921 |
PE anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
575 |
FC
|
| 13-5921 |
Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
|
| 14-5921 |
Affinity Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
IHC
IP
WB
|
| 15-5921 |
PE-Cy5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
670 |
FC
|
| 16-5921 |
Functional Grade* Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
|
| 17-5921 |
APC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
633 |
660 |
FC
|
| 25-5921 |
PE-Cy7 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
760 |
FC
|
| 27-5921 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 35-5921 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
690 |
FC
|
| 36-5921 |
Functional Grade* Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
|
| 45-5921 |
PerCP-Cy5.5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
690 |
FC
|
| 57-5921 |
Pacific Blue® anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
This TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kina, T., K. Ikuta, et al. (2000). "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." Br J Haematol 109(2): 280-87.
Vannucchi, A. M., F. Paoletti, et al. (2000). "Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice."
Blood 95(8): 2559-68.
Related Products
Cat.
45-4031 PerCP-Cy5.5 Rat IgG2b Isotype Control
Mouse Cellular Antigens Chart