Product Information

Contents: PerCP-Cy5.5 anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)
Catalog Number: 45-5892
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: AA4.1
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 bone marrow cells with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.5 μg of PerCP-Cy5.5 Rat IgG2b Isotype Control (cat. 45-4031) (left) or 0.5 μg of PerCP-Cy5.5 anti-mouse CD93 (AA4.1) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5892	Affinity Purified anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	N/A	N/A	FC IHC
13-5892	Biotin anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	N/A	N/A	FC
11-5892	FITC anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	488	518	FC
12-5892	PE anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	488	575	FC
17-5892	APC anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	633	660	FC
25-5892	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	488	760	FC
45-5892	PerCP-Cy5.5 anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen)	488	690	FC

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1⁺ cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1⁺/CD23^-/sIgMhi), T2 (AA4.1⁺/CD23⁺/sIgMhi), and T3 (AA4.1⁺/CD23⁺/sIgMlo). AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This AA4.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This AA4.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Dean YD, McGreal EP, Akatsu H, Gasque P. 2000 Molecular and cellular properties of the rat AA4 antigen, a C-type lectin-like receptor with structural homology to thrombomodulin. J Biol Chem. Nov 3;275(44):34382-92.
McKearn JP, Baum C, Davie JM. 1984 Cell surface antigens expressed by subsets of pre-B cells and B cells. J Immunol. Jan;132(1):332-9.

Related Products

Cat. 45-4031 PerCP-Cy5.5 Rat IgG2b Isotype Control