Also known as: Tbet, Th1-specific T box transcription factor, T-box expressed in T cells
RUO: For Research Use Only
Intracellular staining of the HEK 293T cell line transiently transfected with mouse T-bet with 0.125 µg of Mouse IgG1 κ Isotype Control PerCP-Cy5.5 (cat. 45-4714) (open histogram) or 0.125 µg of Anti-Human/Mouse T-bet PerCP-Cy5.5 (filled histogram). Total cells were used for analysis.
Contents: Anti-Human/Mouse T-bet PerCP-Cy5.5 Catalog Number: 45-5825 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBio4B10 (4B10, 4-B10) Host/Isotype: Mouse IgG1
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Size
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45-5825-80
25 ug
45-5825-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-γ, and T-Bet also regulates the expression of IFN-γ, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-γ, T-Bet is also known to regulate the expression of IL-12Rβ and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio4B10 (4B10, 4-B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This eBio4B10 (4B10, 4-B10) antibody has been tested by flow cytometric analysis of mouse T-Bet-transfected 293T cells. This antibody can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
For intracellular staining with the eBio4B10 monoclonal antibody, it is recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol. Please click here for Staining Protocol (refer to Protocol B: One step protocol for intracellular (nuclear) proteins).
References
Szabo SJ, Kim ST, Costa GL, Zhang X, Fathman CG, Glimcher LH.
A novel transcription factor, T-bet, directs Th1 lineage commitment.
Cell. 2000 Mar 17;100(6):655-69. (4B10, FC, WB, PubMed)
Usui T, Preiss JC, Kanno Y, Yao ZJ, Bream JH, O'Shea JJ, Strober W.
T-bet regulates Th1 responses through essential effects on GATA-3 function rather than on IFNG gene acetylation and transcription.
J Exp Med. 2006 Mar 20;203(3):755-66.
Hwang ES, Hong JH, Glimcher LH.
IL-2 production in developing Th1 cells is regulated by heterodimerization of RelA and T-bet and requires T-bet serine residue 508.
J Exp Med. 2005 Nov 7;202(9):1289-300.
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