Product Information
Contents: PerCP-Cy5.5 anti-human CD209 (DC-SIGN)
Catalog Number: 45-2099
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
Clone: eB-h209
Isotype: Rat IgG2a, κ
HLDA No.: N/A
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Staining of Human monocyte-derived immature dendritic cells (cultured with GM-CSF and IL-4) with 0.25 μg of PerCP-Cy5.5 Rat IgG2a Iso Cntrl (cat. 45-4321) (left) or PerCP-Cy5.5 anti-human CD209 (eB-h209) (right). Total viable cells were used for analysis.
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44 kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eB-h209 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eB-h209 antibody has been pre-titrated and tested by flow cytometric analysis of human dendritic cell cultures. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.
References
eBioscience (2001). Unpublished results.
Geijtenbeek, T.B, D.S. Douglas, et al. (2000) "DC-SIGN, a Dendritic Cell-Specific HIV-1-Binding protein that Enhances trans-Infection of T cells."
Cell 100(5): 587-597.
Geijtenbeek, T.B, R Torensma, et al. (2000). "Identification of DC-SIGN, a Novel Dendritic Cell-Specific ICAM-3 Receptor that Supports Primary Immune Responses."
Cell 100(5): 575-585.
Geijtenbeek, T.B, D.J. Krooshop, et al. (2000). "DC-SIGN-ICAM-2 Interaction Mediates Dendritic Cell Trafficking." Nat. Immunol. 1(4):353-357
Pohlmann, S, F Baribaud, et al. (2001). "DC-SIGN Interactions with Human Immunodeficiency Virus type 1 and 2 and Simian Immunodeficiency Virus." J Virol. 75(10):4664-4672
Related Products
Cat. 45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control