Product Information

Contents: PerCP-Cy5.5 anti-human CD209 (DC-SIGN)
Catalog Number: 45-2099
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eB-h209
Isotype: Rat IgG2a, κ
HLDA No.: N/A

Staining of Human monocyte-derived immature dendritic cells (cultured with GM-CSF and IL-4) with 0.25 μg of PerCP-Cy5.5 Rat IgG2a Iso Cntrl (cat. 45-4321) (left) or PerCP-Cy5.5 anti-human CD209 (eB-h209) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2099	Affinity Purified anti-human CD209 (DC-SIGN)	N/A	N/A	FC IP
13-2099	Biotin anti-human CD209 (DC-SIGN)	N/A	N/A	FC
11-2099	FITC anti-human CD209 (DC-SIGN)	488	518	FC
12-2099	PE anti-human CD209 (DC-SIGN)	488	575	FC
17-2099	APC anti-human CD209 (DC-SIGN)	633	660	FC
45-2099	PerCP-Cy5.5 anti-human CD209 (DC-SIGN) (PE-Cy5.5 replacement)	488	690	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44 kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eB-h209 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eB-h209 antibody has been pre-titrated and tested by flow cytometric analysis of human dendritic cell cultures. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

eBioscience (2001). Unpublished results.
Geijtenbeek, T.B, D.S. Douglas, et al. (2000) "DC-SIGN, a Dendritic Cell-Specific HIV-1-Binding protein that Enhances trans-Infection of T cells." Cell 100(5): 587-597.
Geijtenbeek, T.B, R Torensma, et al. (2000). "Identification of DC-SIGN, a Novel Dendritic Cell-Specific ICAM-3 Receptor that Supports Primary Immune Responses." Cell 100(5): 575-585.
Geijtenbeek, T.B, D.J. Krooshop, et al. (2000). "DC-SIGN-ICAM-2 Interaction Mediates Dendritic Cell Trafficking." Nat. Immunol. 1(4):353-357
Pohlmann, S, F Baribaud, et al. (2001). "DC-SIGN Interactions with Human Immunodeficiency Virus type 1 and 2 and Simian Immunodeficiency Virus." J Virol. 75(10):4664-4672

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Cat. 45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control