Product Information

Contents: PerCP-Cy5.5 anti-human CD209 (DC-SIGN)
Catalog Number: 45-2099
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eB-h209
Isotype: Rat IgG2a, κ
HLDA No.: N/A

Staining of Human monocyte-derived immature dendritic cells (cultured with GM-CSF and IL-4) with 0.25 μg of PerCP-Cy5.5 Rat IgG2a Iso Cntrl (cat. 45-4321) (left) or PerCP-Cy5.5 anti-human CD209 (eB-h209) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-2099	FITC anti-human CD209 (DC-SIGN)	488	518	FC
12-2099	PE anti-human CD209 (DC-SIGN)	488	575	FC
13-2099	Biotin anti-human CD209 (DC-SIGN)	N/A	N/A	FC
14-2099	Affinity Purified anti-human CD209 (DC-SIGN)	N/A	N/A	FC IP
17-2099	APC anti-human CD209 (DC-SIGN)	633	660	FC
45-2099	PerCP-Cy5.5 anti-human CD209 (DC-SIGN)	488	690	FC

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44 kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eB-h209 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eB-h209 antibody has been pre-titrated and tested by flow cytometric analysis of human dendritic cell cultures. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

eBioscience (2001). Unpublished results.
Geijtenbeek, T.B, D.S. Douglas, et al. (2000) "DC-SIGN, a Dendritic Cell-Specific HIV-1-Binding protein that Enhances trans-Infection of T cells." Cell 100(5): 587-597.
Geijtenbeek, T.B, R Torensma, et al. (2000). "Identification of DC-SIGN, a Novel Dendritic Cell-Specific ICAM-3 Receptor that Supports Primary Immune Responses." Cell 100(5): 575-585.
Geijtenbeek, T.B, D.J. Krooshop, et al. (2000). "DC-SIGN-ICAM-2 Interaction Mediates Dendritic Cell Trafficking." Nat. Immunol. 1(4):353-357
Pohlmann, S, F Baribaud, et al. (2001). "DC-SIGN Interactions with Human Immunodeficiency Virus type 1 and 2 and Simian Immunodeficiency Virus." J Virol. 75(10):4664-4672

Related Products

Cat. 45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control