mouse/rat CD90.1 (Thy-1.1, Thy1.1)

Contents: PerCP-Cy5.5 anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)
Catalog Number: 45-0900
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS51
Isotype: Mouse IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
45-0900-80	25 ug	$100

*International customers: Please contact your distributor for region specific pricing. May not be available for sale in all countries (see below).

Staining of rat thymocytes with 0.03 μg of PerCP-Cy5.5 Mouse IgG2a, K Iso Cntrl (cat. 45-4724) (open histogram) or 0.03 μg of PerCP-Cy5.5 anti-mouse/rat CD90.1 (HIS51) (colored histogram).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0900	FITC anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	518	FC
12-0900	PE anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	575	FC
13-0900	Biotin anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	N/A	N/A	FC
14-0900	Affinity Purified anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	N/A	N/A	FC IH/F
15-0900	PE-Cy5 anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	670	FC
17-0900	APC anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	633	660	FC
25-0900	Phycoerythrin-Cy7 (PE-Cy7) anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	760	FC
45-0900	PerCP-Cy5.5 anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)	488	690	FC
57-0900	Pacific Blue® anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)	402	421	FC

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HIS51 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HIS51 antibody has been tested by flwo cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kroese, F. G., N. K. de Boer, et al. 1995. Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues. Cell Immunol 162(2): 185-93.
Hosseinzadeh, H. and I. Goldschneider 1993. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol 150(5): 1670-9.
Ishizu, A., H. Ishikura, et al. 1995. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int Immunol 7(12): 1939-47.
Hermans, M. H. and D. Opstelten 1991. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem 39(12): 1627-34.

Related Products

Cat. 45-4724 PerCP-Cy5.5 Mouse IgG2a, K Isotype Control