Contents: PerCP-Cy5.5 anti-mouse CD69 (Very Early Activation Antigen, VEA)
Catalog Number: 45-0691
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: H1.2F3
Isotype: Armenian Hamster IgG
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
May not be available for sale in all countries (see below).
|
|
Staining of 1 day ConA activated BALB/c splenocytes with 0.25 μg of PerCP-Cy5.5 Armenian Hamster IgG Iso Cntrl (cat. 45-4888) (open histogram) or 0.25 μg of PerCP-Cy5.5 anti-mouse CD69 (H1.2F3) (colored histogram). Total viable cells were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0691 |
FITC anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
518 |
FC
|
| 12-0691 |
PE anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
575 |
FC
|
| 15-0691 |
PE-Cy5 anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
670 |
FC
|
| 17-0691 |
Allophycocyanin (APC) anti-mouse CD69 (Very Early Activation Antigen, VEA) |
633 |
660 |
FC
|
| 25-0691 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
760 |
FC
|
| 45-0691 |
PerCP-Cy5.5 anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
690 |
FC
|
| 93-0691 |
eFluorTM 605NC anti-mouse CD69 (Very Early Activation Antigen, VEA) |
350-500 |
605 |
FC
|
| 14-0691 |
Affinity Purified anti-mouse CD69 (Very Early Activation Antigen, VEA) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 16-0691 |
Functional Grade* Purified anti-mouse CD69 (Very Early Activation Antigen, VEA) |
N/A |
N/A |
FA
FC
|
| 13-0691 |
Biotin anti-mouse CD69 (Very Early Activation Antigen, VEA) |
N/A |
N/A |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This H1.2F3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This H1.2F3 antibody has been tested by flow cytometric analysis of activated splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Yokoyama, W. M., F. Koning, et al. (1988). "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation."
J Immunol 141(2): 369-76.
Related Products
Cat.
45-4888 PerCP-Cy5.5 Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Mouse Cellular Antigens Chart