Product Information

Contents: PerCP-Cy5.5 anti-human CD45 (LCA)
Catalog Number: 45-0459
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI30
Isotype: Mouse IgG1, κ
HLDA No.: IV N816

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or 0.125 μg of PerCP-Cy5.5 anti-human CD45 (HI30) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0459	FITC anti-human CD45 (LCA)	488	518	FC
12-0459	PE anti-human CD45 (LCA)	488	575	FC
13-0459	Biotin anti-human CD45 (LCA)	N/A	N/A	FC
14-0459	Affinity Purified anti-human CD45 (LCA)	N/A	N/A	FC IHC
15-0459	PE-Cy5 anti-human CD45 (LCA)	488	670	FC
17-0459	Allophycocyanin (APC) anti-human CD45 (LCA)	633	660	FC
19-0459	Cy5 anti-human CD45 (LCA)	633	670	FC
25-0459	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45 (LCA)	488	760	FC
27-0459	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD45 (LCA) (APC-Cy7 Equivalent)	633	779	FC
35-0459	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD45 (LCA)	488	690	FC
45-0459	PerCP-Cy5.5 anti-human CD45 (LCA)	488	690	FC
57-0459	Pacific Blue® anti-human CD45 (LCA)	402	421	FC

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 45-0451 PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (clone 30-F11)
Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control