Contents: PerCP-Cy5.5 anti-human CD45 (LCA)
Catalog Number: 45-0459
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI30
Isotype: Mouse IgG1, κ
HLDA No.: IV N816
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Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or 0.125 μg of PerCP-Cy5.5 anti-human CD45 (HI30) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This HI30 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
45-0451 PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (clone 30-F11)
Cat.
45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart