Product Information

Contents: PerCP-Cy5.5 anti-human CD45RA
Catalog Number: 45-0458
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906

Staining of normal human peripheral blood cells with 0.06 μg of PerCP-Cy5.5 Mouse IgG2b Iso Cntrl (cat. 45-4732) (open histogram) or PerCP-Cy5.5 anti-human CD45RA (HI100) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC IHC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA	488	690	FC
57-0458	Pacific Blue® anti-human CD45RA	402	421	FC

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.06 μg) per million cells in a 100 μl total staining volume.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 45-4732 PerCP-Cy5.5 Mouse IgG2b Isotype Control