Product Information
Contents: PerCP-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)
Catalog Number: 45-0453
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A20
Isotype: Mouse IgG2a, κ
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Staining of SJL splenocytes with 0.25 μg of PerCP-Cy5.5 Mouse IgG2a, K Iso Cntrl (cat. 45-4724) (open histogram) or 0.125 μg of PerCP-Cy5.5 anti-mouse CD45.1 (A20) (colored histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0453 |
Affinity Purified anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
N/A |
N/A |
FC
IP
|
| 13-0453 |
Biotin anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
N/A |
N/A |
FC
|
| 11-0453 |
FITC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
518 |
FC
|
| 12-0453 |
PE anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
575 |
FC
|
| 15-0453 |
PE-Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
670 |
FC
|
| 17-0453 |
APC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
633 |
660 |
FC
|
| 19-0453 |
Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
633 |
670 |
FC
|
| 25-0453 |
PE-Cy7 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
760 |
FC
|
| 35-0453 |
PE-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
690 |
FC
|
| 45-0453 |
PerCP-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) (PE-Cy5.5 replacement) |
488 |
690 |
FC
|
| 93-0453 |
eFluorTM 605NC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
350-500 |
605 |
FC
|
| 47-0453 |
Allophycocyanin-eFluorTM 780 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
| 48-0453 |
eFluorTM 450 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) (Pacific Blue® replacement) |
405 |
450 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This A20 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This A20 antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.
Related Products
Cat. 45-4724 PerCP-Cy5.5 Mouse IgG2a, K Isotype Control
