Contents: PerCP-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)
Catalog Number: 45-0453
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A20
Isotype: Mouse IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
May not be available for sale in all countries (see below).
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Staining of SJL splenocytes with 0.25 μg of PerCP-Cy5.5 Mouse IgG2a, K Iso Cntrl (cat. 45-4724) (open histogram) or 0.125 μg of PerCP-Cy5.5 anti-mouse CD45.1 (A20) (colored histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0453 |
FITC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
518 |
FC
|
| 12-0453 |
PE anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
575 |
FC
|
| 13-0453 |
Biotin anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
N/A |
N/A |
FC
|
| 14-0453 |
Affinity Purified anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
N/A |
N/A |
FC
IP
|
| 15-0453 |
PE-Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
670 |
FC
|
| 17-0453 |
APC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
633 |
660 |
FC
|
| 19-0453 |
Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
633 |
670 |
FC
|
| 25-0453 |
PE-Cy7 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
760 |
FC
|
| 27-0453 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
633 |
779 |
FC
|
| 35-0453 |
PE-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
690 |
FC
|
| 45-0453 |
PerCP-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) |
488 |
690 |
FC
|
PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This A20 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This A20 antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.
Related Products
Cat.
45-4724 PerCP-Cy5.5 Mouse IgG2a, K Isotype Control
Mouse Cellular Antigens Chart