Contents: PerCP-Cy5.5 anti-mouse/human CD45R (B220)
Catalog Number: 45-0452
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RA3-6B2
Isotype: Rat IgG2a, κ
HLDA No.: N/A
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May not be available for sale in all countries (see below).
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Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.25 μg of PerCP-Cy5.5 Rat IgG2a Iso Cntrl (cat. 45-4321) (left) or 0.25 μg of PerCP-Cy5.5 anti-mo/hu B220 (RA3-6B2) (right). Cells in the lymphocyte gate were used for analysis.
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PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the
lpr/lpr mutant mouse also express this antigen.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This RA3-6B2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Morse, H. C. d., W. F. Davidson, et al. (1982). "Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset."
J Immunol 129(6): 2612-5.
Coffman, R. L. (1982). "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development."
Immunol Rev 69: 5-23.
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