Contents: PerCP-Cy5.5 anti-human CD33
Catalog Number: 45-0338
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: WM-53 (WM53)
Isotype: Mouse IgG1, κ
HLDA No.: VI M505
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Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or PerCP-Cy5.5 anti-human CD33 (WM-53) (colored histogram). Cells in the monocyte gate were used for analysis.
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PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This WM-53 (WM53) antibody has been pre-titrated and tested by fllow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.
References
Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC. 1987 Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 5(4):215-25.
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Human Cellular Antigens Chart