Product Information

Contents: PerCP-Cy5.5 anti-human CD28 (costimulatory)
Catalog Number: 45-0289
Sizes: 25 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CD28.2
Isotype: Mouse IgG1, κ
HLDA No.: V 5T CD28.05

Staining of normal human peripheral blood cells with 0.5 μg of PerCP-Cy5.5 Mouse IgG1,K Iso Cntrl (cat. 45-4714) (open histogram) or PerCP-Cy5.5 anti-human CD28 (CD28.2) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0289	FITC anti-human CD28 (costimulatory)	488	518	FC
12-0289	PE anti-human CD28 (costimulatory)	488	575	FC
13-0289	Biotin anti-human CD28 (costimulatory)	N/A	N/A	FC
14-0289	Affinity Purified anti-human CD28 (costimulatory)	N/A	N/A	FA FC IHC IP
16-0289	Functional Grade* Purified anti-human CD28 (costimulatory)	N/A	N/A	FA FC
17-0289	APC anti-human CD28 (costimulatory)	633	660	FC
25-0289	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD28 (costimulatory)	488	760	FC
45-0289	PerCP-Cy5.5 anti-human CD28 (costimulatory)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CD28.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysi sof normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Schlossman, S., L. Bloumsell, et al. eds. 1995.Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 45-0281 PerCP-Cy5.5 anti-mouse CD28 (clone 37.51)
Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control