Product Information
Contents: PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)
Catalog Number: 45-0259
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072
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Staining of normal human peripheral blood cells with APC anti-human CD4 (RPA-T4) (cat. 17-0049) and staining buffer (autofluorescence) (left) or PerCP-Cy5.5 anti-human CD25 (BC96) (right). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0259 |
Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 13-0259 |
Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 12-0259 |
PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
575 |
FC
|
| 15-0259 |
Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
670 |
FC
|
| 17-0259 |
Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
633 |
660 |
FC
|
| 25-0259 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
760 |
FC
|
| 53-0259 |
Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
519 |
FC
|
| 45-0259 |
PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) (PE-Cy5.5 replacement) |
488 |
690 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This BC96 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.
References
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control
