Contents: PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)
Catalog Number: 45-0259
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
May not be available for sale in all countries (see below).
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Staining of normal human peripheral blood cells with APC anti-human CD4 (RPA-T4) (cat. 17-0049) and staining buffer (autofluorescence) (left) or PerCP-Cy5.5 anti-human CD25 (BC96) (right). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0259 |
**May be discontinued; see superior product Alexa Fluor® 488** FITC anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
518 |
FC
|
| 12-0259 |
PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
575 |
FC
|
| 13-0259 |
Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 14-0259 |
Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 15-0259 |
Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
670 |
FC
|
| 17-0259 |
Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
633 |
660 |
FC
|
| 25-0259 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
760 |
FC
|
| 45-0259 |
PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
690 |
FC
|
| 53-0259 |
Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
519 |
FC
|
PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This BC96 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.
References
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart