mouse CD24 (HAS, Heat Stable Antigen)

Contents: PerCP-Cy5.5 anti-mouse CD24 (Heat Stable Antigen)
Catalog Number: 45-0242
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: M1/69
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
45-0242-80	25 ug	$125
45-0242-82	100 ug	$295

*International customers: Please contact your distributor for region specific pricing. May not be available for sale in all countries (see below).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0242	FITC anti-mouse CD24 (Heat Stable Antigen)	488	518	FC
12-0242	PE anti-mouse CD24 (Heat Stable Antigen)	488	575	FC
15-0242	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD24 (Heat Stable Antigen)	488	670	FC
57-0242	Pacific Blue® anti-mouse CD24 (Heat Stable Antigen)	402	421	FC
45-0242	PerCP-Cy5.5 anti-mouse CD24 (Heat Stable Antigen)	488	690	FC
93-0242	eFluor^TM 605^NC anti-mouse CD24 (Heat Stable Antigen)	350-500	605	FC
14-0242	Affinity Purified anti-mouse CD24 (Heat Stable Antigen)	N/A	N/A	FA FC IHC WB
16-0242	Functional Grade* Purified anti-mouse CD24 (Heat Stable Antigen)	N/A	N/A	FA FC
13-0242	Biotin anti-mouse CD24 (Heat Stable Antigen)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This M1/69 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.03 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Springer T, Galfre G, Secher DS, Milstein C. 1978. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 8(8):539-51.
Alterman, L. A., I. N. Crispe, et al. 1990. Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies. Eur J Immunol 20(7): 1597-602.
Wilson, A., L. M. Day, et al. 1988. Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen. Cell Immunol 117(2): 312-26.
Hunte BE, Capone M, Zlotnik A, Rennick D, Moore TA. 1998. Acquisition of CD24 expression by Lin-CD43+B220(low)ckit(hi) cells coincides with commitment to the B cell lineage. Eur J Immunol. 28(11):3850-6.

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