Product Information

Contents: PerCP-Cy5.5 anti-human CD20
Catalog Number: 45-0209
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2H7
Isotype: Mouse IgG2b, κ
HLDA No.: IV B201

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or PerCP-Cy5.5 anti-human CD20 (2H7) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0209	FITC anti-human CD20	488	518	FC
12-0209	PE anti-human CD20	488	575	FC
13-0209	Biotin anti-human CD20	N/A	N/A	FC IH/F
14-0209	Affinity Purified anti-human CD20	N/A	N/A	FC IH/F
15-0209	PE-Cy5 anti-human CD20	488	670	FC
17-0209	Allophycocyanin (APC) anti-human CD20	633	660	FC
25-0209	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD20	488	760	FC
27-0209	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD20 (APC-Cy7 Equivalent)	633	779	FC
45-0209	PerCP-Cy5.5 anti-human CD20	488	690	FC
56-0209	Alexa Fluor® 700 anti-human CD20 (APC-Cy5.5 Equivalent)	633	723	FC
57-0209	Pacific Blue® anti-human CD20	402	421	FC

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Reinherz, E.L., et al. eds. 1985. Leukocyte Typing II (Vol. I, II, and III). Human Leukocyte Differentiation Antigens detected by Monoclonal Antibodies.
McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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Cat. 45-4732 PerCP-Cy5.5 Mouse IgG2b Isotype Control