mouse CD14

Contents: PerCP-Cy5.5 anti-mouse CD14
Catalog Number: 45-0141
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: Sa2-8
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
45-0141-80	25 ug	$125
45-0141-82	100 ug	$300

*International customers: Please contact your distributor for region specific pricing. May not be available for sale in all countries (see below).

Staining of thioglycolate-induced PEC with 0.25 μg of PerCP-Cy5.5 Rat IgG2a Iso Cntrl (cat. 45-4321) (open histogram) or 0.25 μg of PerCP-Cy5.5 anti-mouse CD14 (Sa2-8) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0141	Fluorescein (FITC) anti-mouse CD14	488	518	FC
12-0141	Phycoerythrin (PE) anti-mouse CD14	488	575	FC
13-0141	Biotin anti-mouse CD14	N/A	N/A	FC
14-0141	Affinity Purified anti-mouse CD14	N/A	N/A	FC IP WB
16-0141	Functional Grade* Purified anti-mouse CD14	N/A	N/A	FC
17-0141	Allophycocyanin (APC) anti-mouse CD14	633	660	FC
45-0141	PerCP-Cy5.5 anti-mouse CD14	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-κB activation or TNF-α production with LPS stimulation).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This Sa2-8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This Sa2-8 antibody has been tested by flow cytometric analysis of thioglycolate elicited peritoneal cells (PECs). This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Akashi S, Saitoh S, Wakabayashi Y, Kikuchi T, Takamura N, Nagai Y, Kusumoto Y, Fukase K, Kusumoto S, Adachi Y, Kosugi A, Miyake K. 2003. Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14. J Exp Med. 198(7): 1035-42.

Related Products

Cat. 45-0149 PerCP-Cy5.5 anti-human CD14 (clone 61D3)
Cat. 45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control