mouse CD11c (Integrin aX, p150/90)

Contents: PerCP-Cy5.5 anti-mouse CD11c (Integrin aX, p150/90)
Catalog Number: 45-0114
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: N418
Isotype: Armenian Hamster IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
45-0114-80	25 ug	$125
45-0114-82	100 ug	$300

*International customers: Please contact your distributor for region specific pricing. May not be available for sale in all countries (see below).

Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.125 μg of PerCP-Cy5.5 Armenian Hamster IgG Iso Cntrl (cat. 45-4888) (left) or 0.125 μg of PerCP-Cy5.5 anti-mouse CD11c (N418) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0114	FITC anti-mouse CD11c (Integrin aX, p150/90)	488	518	FC
12-0114	PE anti-mouse CD11c (Integrin aX, p150/90)	488	575	FC
13-0114	Biotin anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC
14-0114	Affinity Purified anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC IH/F IP
15-0114	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD11c (Integrin aX, p150/90)	488	670	FC
16-0114	Functional Grade* Purified anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC
17-0114	APC anti-mouse CD11c (Integrin aX, p150/90)	633	660	FC
25-0114	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD11c (Integrin aX, p150/90)	488	760	FC
27-0114	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD11c (Integrin aX, p150/90) (APC-Cy7 Equivalent)	633	779	FC
35-0114	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD11c (Integrin aX, p150/90)	488	690	FC
45-0114	PerCP-Cy5.5 anti-mouse CD11c (Integrin aX, p150/90)	488	690	FC
51-0114	Alexa Fluor® 647 anti-mouse CD11c (Integrin aX, p150/90)	633	668	FC
53-0114	Alexa Fluor® 488 anti-mouse CD11c (Integrin aX, p150/90)	488	519	FC
56-0114	Alexa Fluor® 700 anti-mouse CD11c (Integrin aX, p150/90) (APC-Cy5.5 Equivalent)	633	723	FC
57-0114	Pacific Blue® anti-mouse CD11c (Integrin aX, p150/90)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The N418 monoclonal antibody reacts with mouse CD11c, the integrin α_X. CD11c non-covalently associates with β₂ integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This N418 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Crowley MT, Inaba K, Witmer-Pack MD, Gezelter S, Steinman RM. 1990. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Methods. 133:55-66.

Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. 1990. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 171:1753-71.

Esche C, Gambotto A, Satoh Y, Gerein V, Robbins PD, Watkins SC, Lotze MT, Shurin MR. 1999. CD154 inhibits tumor-induced apoptosis in dendritic cells and tumor growth. Eur J Immunol. 29:2148-55.

Finkelman FD, Lees A, Birnbaum R, Gause WC, Morris SC. 1996. Dendritic cells can present antigen in vivo in a tolerogenic or immunogenic fashion. J Immunol. 157:1406-14.

Related Products

Cat. 45-4888 PerCP-Cy5.5 Armenian Hamster IgG Isotype Control (clone eBio299Arm)