Product Information
Contents: PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain)
Catalog Number: 45-0088
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
Clone: RPA-T8
Isotype: Mouse IgG1, κ
HLDA No.: IV T171
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Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or 0.25 μg of PerCP-Cy5.5 anti-human CD8 (RPA-T8) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This RPA-T8 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.
Related Products
Cat. 45-0081 PerCP-Cy5.5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (clone 53-6.7)Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control