Product Information

Contents: PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain)
Catalog Number: 45-0088
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RPA-T8
Isotype: Mouse IgG1, κ
HLDA No.: IV T171

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or 0.25 μg of PerCP-Cy5.5 anti-human CD8 (RPA-T8) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0088	FITC anti-human CD8 (CD8a; alpha chain)	488	518	FC
12-0088	PE anti-human CD8 (CD8a; alpha chain)	488	575	FC
14-0088	Affinity Purified anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC IH/F
15-0088	PE-Cy5 anti-human CD8 (CD8a; alpha chain)	488	670	FC
17-0088	APC anti-human CD8 (CD8a; alpha chain)	633	660	FC
25-0088	PE-Cy7 anti-human CD8 (CD8a; alpha chain)	488	760	FC
27-0088	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD8 (CD8a; alpha chain) (APC-Cy7 Equivalent)	633	779	FC
45-0088	PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain)	488	690	FC

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RPA-T8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

Related Products

Cat. 45-0081 PerCP-Cy5.5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (clone 53-6.7)
Cat. 45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control