mouse CD8a (Ly-2, Ly2)

Contents: PerCP-Cy5.5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)
Catalog Number: 45-0081
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 53-6.7
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
45-0081-80	25 ug	$100
45-0081-82	100 ug	$300

*International customers: Please contact your distributor for region specific pricing. May not be available for sale in all countries (see below).

Staining of C57Bl/6 splenocytes with 0.5 μg of PerCP-Cy5.5 Rat IgG2a Iso Cntrl (cat. 45-4321) (open histogram) or 0.125 μg of PerCP-Cy5.5 anti-mouse CD8 (53-6.7) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0081	FITC anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	518	FC
12-0081	PE anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	575	FC
13-0081	Biotin anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FC
14-0081	Affinity Purified anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FC IH/F IP
15-0081	PE-Cy5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	670	FC
16-0081	Functional Grade* Purified anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FC
17-0081	APC anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	633	660	FC
19-0081	Cy5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	633	670	FC
25-0081	PE-Cy7 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	760	FC
27-0081	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (APC-Cy7 Equivalent)	633	779	FC
36-0081	Functional Grade* Biotin anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	N/A	N/A	FC IH/F
45-0081	PerCP-Cy5.5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	690	FC
51-0081	Alexa Fluor® 647 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	633	668	FC
53-0081	Alexa Fluor® 488 anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	488	519	FC
56-0081	Alexa Fluor® 700 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (APC-Cy5.5 Equivalent)	633	723	FC
57-0081	Pacific Blue® anti-mouse CD8 (alpha subunit, CD8a, Ly-2)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature αβ TCR T cells express CD8 αβ while γδ TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 αα. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 53-6.7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.
Ledbetter, J. A., R. V. Rouse, et al. (1980). "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." J Exp Med 152(2): 280-95.

Related Products

Cat. 45-0088 PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain) (clone RPA-T8)
Cat. 45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control