Contents: PerCP-Cy5.5 anti-mouse CD5 (Ly-1)
Catalog Number: 45-0051
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 53-7.3
Isotype: Rat IgG2a, κ
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Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.25 μg of PerCP-Cy5.5 Rat IgG2a Iso Cntrl (cat. 45-4321) (left) or 0.06 μg of PerCP-Cy5.5 anti-mouse CD5 (53-7.3) (right). Total viable cells were used for analysis.
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PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ledbetter, J. A. and L. A. Herzenberg. 1979. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 63-90.
Ledbetter, J. A., R. V. Rouse, et al. 1980. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152(2): 280-95.
Related Products
Cat.
45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart