Product Information

Contents: PerCP-Cy5.5 anti-mouse CD4 (L3T4)
Catalog Number: 45-0042
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RM4-5
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 μg of PerCP-Cy5.5 anti-mouse CD4 (RM4-5) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0042	FITC anti-mouse CD4 (L3T4)	488	518	FC
12-0042	PE anti-mouse CD4 (L3T4)	488	575	FC
13-0042	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC
14-0042	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FA FC IH/F
15-0042	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC
16-0042	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC
17-0042	APC anti-mouse CD4 (L3T4)	633	660	FC
25-0042	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC
27-0042	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD4 (L3T4) (APC-Cy7 Equivalent)	633	779	FC
30-0042	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse CD4 (L3T4) (Alexa Fluor® 700 Equivalent)	633	690	FC
35-0042	PE-Cy5.5 anti-mouse CD4 (L3T4)	488	690	FC
45-0042	PerCP-Cy5.5 anti-mouse CD4 (L3T4)	488	690	FC
56-0042	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC
57-0042	Pacific Blue® anti-mouse CD4 (L3T4)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RM4-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Okumura, K. 2000. Personal communication.
Wilde, D. B., P. Marrack, et al. 1983. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol 131(5): 2178-83.

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only.

Related Products

Cat. 45-0049 PerCP-Cy5.5 anti-human CD4 (clone RPA-T4)
Cat. 45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control