Product Information

Contents: PerCP-Cy5.5 anti-mouse CD3 (epsilon subunit; CD3e)
Catalog Number: 45-0031
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 145-2C11
Isotype: Armenian Hamster IgG

Staining of BALB/c splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and staining buffer (autofluorescence) (left) or 0.5 μg of PerCP-Cy5.5 anti-mouse CD3 (145-2C11) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0031	FITC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	518	FC
12-0031	PE Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	575	FC
13-0031	Biotin Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FC
14-0031	Affinity Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
15-0031	PE-Cy5 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	670	FC
16-0031	Functional Grade* Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC
17-0031	APC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	633	660	FC
25-0031	PE-Cy7 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	760	FC
35-0031	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD3 (epsilon subunit; CD3e)	488	690	FC
36-0031	Functional Grade* Biotin anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
45-0031	PerCP-Cy5.5 anti-mouse CD3 (epsilon subunit; CD3e)	488	690	FC
53-0031	Alexa Fluor® 488 anti-mouse CD3 (epsilon subunit; CD3e)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

PerCP contained in this product is protected by patents owned by Becton, Dickinson & Company (European patent 0314406, or Japanese Patent JP1888759). This product will not be sold or shipped to customers in France, Germany, Italy, United Kingdom or Japan until the pertinent patents are no longer in effect (October 21, 2008).

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 145-2C11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes.

References

Leo O, Foo M, et al. 1987. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci U S A 84(5): 1374-8.

Samelson LE, O'Shea JJ, et al. 1987. T cell antigen receptor phosphorylation induced by an anti-receptor antibody. J Immunol 139(8): 2708-14.

Kearse KP. 1998. Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):14152-7. (IP and WB, PubMed)

Schuchert MJ, Wright RD, et al. 2000. Characterization of a newly discovered T-cell receptor beta-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment. Nat Med. 2000 Aug;6(8):904-9. (WB and IP, PubMed)

Payer E, Elbe A, and Stingl G. 1991. Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells. J Immunol. 1991 Apr 15;146(8):2536-43. (IHC frozen, PubMed)

Podd BS, Thoits J, et al. 2006. T cells in cryptopatch aggregates share TCR gamma variable region junctional sequences with gamma delta T cells in the small intestinal epithelium of mice. J Immunol. 176(11):6532-42. (IHC frozen, PubMed)

Geng H, Zhang GM, et al. 2006. Soluble form of T cell Ig mucin 3 is an inhibitory molecule in T cell-mediated immune response. J Immunol. 176(3):1411-20. (IHC paraffin, PubMed)

Related Products

Cat. 45-4888 PerCP-Cy5.5 Armenian Hamster IgG Isotype Control (clone eBio299Arm)