Also known as: Transforming Growth Factor beta 1, TGFbeta1, TGF-b1
RUO: For Research Use Only
Contents: Human TGF beta 1 Single-Use ELISA RSG Standard Catalog Number: 39-8348 Formulation: Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA, 0.09% NaN3. 0.22 µm filtered. Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent. Source: Human TGFb1 was expressed in CHO cells. Mature TGFb1 (ala279 - ser390; accession #BC001180) was purified from the conditioned media. Purity: Greater than 98%, as determined by SDS-PAGE
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39-8348-65
4 pack
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
Human TGF-β1 (Transforming Growth Factor-β1) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-β1 is a 25 kDa protein with each subunit containing 112 aa residues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human TGFb1 is biologically active. The intended use of the Recombinant Human TGFb1 Single-Use ELISA RSG Standard is as a standard for human TGFb1 Ready-SET-Go! ELISA and human TGFb1 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 ul of standard solution to 2.5 ml of assay diluent to prepare the top standard solution (8000 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 2.5 ml of assay diluent to prepare the top standard solution (8000 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the human TGFb1 ELISA pair.
References
Kotenko, S., et al. 2003. IFN-lambdas mediate anti-viral protection through a distinct class II cytokine receptor complex. Nature Immunol. 4: 69-77.
Mennechet, F., et al. 2006. IFN-lambda-treated dendritic cells specifically induce proliferation of FOXP3-expressing suppressor T cells. Immunobiology. 107: 4417-4423.
Sheppard, P., et al. 2003. IL-28, IL-29, and their class II cytokine receptor IL-28R. Nature Immunol. 4: 63-68.
