Product Information

Contents: Recombinant Mouse GM-CSF Single-Use ELISA RSG Standard
Catalog Number: 39-8331
Sizes: 4 pack
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed mature mouse GM-CSF (accession # NM_009969)
Purity: Greater than 98%, as determined by SDS-PAGE

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8331	Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	BA ELISA std
34-8331	Carrier-Free Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	BA ELISA
39-8331	Single-Use ELISA RSG Standard Recombinant Mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF) is an ~14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-α, IL-2, IL-1, and IFN-γ. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant Mouse GM-CSF is biologically active. The intended use of the Recombinant Mouse GM-CSF Single-Use ELISA RSG Standard is as a standard for mouse GM-CSF Ready-SET-Go! ELISA and mouse GM-CSF matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse GM-CSF ELISA pair (MP1-22E9/MP1-31G6).