Contents: Recombinant Human TNF-α Single-Use ELISA RSG Standard
Catalog Number: 39-8329
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids Val 77-Leu 233 of human TNF-α (accession # HUMTNFAB).
Purity: Greater than 98%, as determined by SDS-PAGE
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Description
Human TNF-α is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4
+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human TNF-α is biologically active. The intended use of the Recombinant Human TNF-α Single-Use ELISA RSG Standard is as a standard for human TNF-α Ready-SET-Go! ELISA and human TNF-α matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the human TNF-α ELISA pair (MAb1/MAb11).
