Single-Use ELISA RSG Standard Recombinant Human IFN-alpha1
RUO: For Research Use Only
Human IFN-α1 Ready-SET-Go! ELISA Standard Curve
Contents: Single-Use ELISA RSG Standard Recombinant Human IFN-alpha1 Catalog Number: 39-8318 Formulation: Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA, 0.09% NaN3. 0.22 µm filtered. Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard. Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent. Source: E. coli expressed amino acids Cys 24-Asp 189 of mature human IFNα1 (accession # NM_024013). Purity: Greater than 98%, as determined by SDS-PAGE
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39-8318-65
4 pack
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
Human IFN-α1 is a type I interferon previously known as B-cell interferon, leukocyte interferon, lymphoblast interferon, and pH2-stable interferon. IFN-α1 is one of at least 23 different known variants of IFN-α. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. The IFN-α forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and other cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances (n-butyrate, 5-bromodeoxyuridine). Human IFN-α1 promotes antiviral, antiparasitic, and antiproliferative activities.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human IFN-α1 is biologically active. The Recombinant Human IFN-α1 Single-Use ELISA RSG Standard is intended to be used as a standard for the Human IFN-α1 Ready-SET-Go! ELISA. Typically, the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 ul of standard solution to 2.5 ml of assay diluent to prepare the top standard solution (2000 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute only as suggested above or recovery may not be accurate. Use within 6 hours after initial dilution.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the Human IFN-α1 ELISA Ready-SET-Go! Set (eBioscience Cat. No. 88-7389)
References
Nagai, T., et al. 2003. Timing of IFN-beta exposure during human dendritic cell maturation and naive Th cell stimulation has contrasting effects on Th1 subset generation: a role for IFN-beta-mediated regulation of IL-12 family cytokines and IL-18 in naive Th cell differentiation. J. Immunol. 171: 5233-43.
