Product Information

Contents: Recombinant Mouse IFN-γ Single-Use ELISA RSG Standard
Catalog Number: 39-8311
Sizes: 4 pack
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids His 23-Cys 155 of mouse IFN-γ (accession # NM_008337).
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8311	Recombinant Mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	BA ELISA std
34-8311	Carrier-Free Recombinant Mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	BA ELISA
39-8311	Single-Use ELISA RSG Standard Recombinant Mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse IFN-γ is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IFN-γ is biologically active. The intended use of the Recombinant Mouse IFN-γ Single-Use ELISA RSG Standard is as a standard for mouse IFN-γ Ready-SET-Go! ELISA and mouse IFN-γ matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use (XMG1.2/R4-6A2 assays):
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (2000 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (2000 pg/ml).

Instructions for use (AN-18/R4-6A2 assays):
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare 2000 pg/ml solution. Mix thoroughly. Further dilute by adding 50 ul of this solution to 0.95 ml of assay diluent to make the top standard solution (100 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare 2000 pg/ml solution. Mix thoroughly. Further dilute by adding 100 ul of this solution to 0.90 ml of assay diluent to make the top standard solution (200 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IFN-γ ELISA pair (XMG1.2/R4-6A2 or AN-18/R4-6A2). These two different assays have different dynamic ranges.