Product Information

Contents: Recombinant Mouse IL-15 Single-Use ELISA RSG Standard
Catalog Number: 39-8151
Sizes: 4 pack
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli
Purity: Greater than 98%, as determined by SDS-PAGE

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
34-8151	Carrier-Free Recombinant Mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	BA ELISA std
39-8151	Single-Use ELISA RSG Standard Recombinant Mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	ELISA
14-8151	Recombinant Mouse IL-15 (Interleukin-15, IL15)	N/A	N/A	BA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse IL-15 is a 14-15 kDa factor, previously known as IL-T. IL-15 is produced by a variety of cells and has a role in the T cell-mediated immune response and the central nervous system. IL-15 binds to the β and γ subunits of the IL-2 receptor; however, these two cytokines have distinct biological activities. IL-15 is a specific maturation factor for NK cells and mimics the BM microenvironment for NK cell development in vitro. It is reported that IL-15 delivers an anti-apoptotic and survival signal for NK, T, and B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-15 is biologically active. The intended use of the Recombinant Mouse IL-15 Single-Use ELISA RSG Standard is as a standard for mouse IL-15 Ready-SET-Go! ELISA and mouse IL-15 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 ul of standard solution to 2.5 ml of assay diluent to prepare the top standard solution (8000 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-15 ELISA pair (eBioMTZ4515/goat polyclonal antibody).