mouse IL-13 (Interleukin-13)

Contents: Recombinant Mouse IL-13 Single-Use ELISA RSG Standard
Catalog Number: 39-8131
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids Ser 26-Phe 131 of mature mouse IL-13 (accession # NM_008355).
Purity: Greater than 98%, as determined by SDS-PAGE

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
39-8131-65	4 pack	$80

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8131	Recombinant Mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	BA ELISA
34-8131	Carrier-Free Recombinant Mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	BA ELISA
39-8131	Single-Use ELISA RSG Standard Recombinant Mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Rα chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-13 is biologically active. The intended use of the Recombinant Mouse IL-13 Single-Use ELISA RSG Standard is as a standard for mouse IL-13 Ready-SET-Go! ELISA and mouse IL-13 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-13 ELISA pair (eBio13A/eBio1316H).

References

de Waal Melfyt, R. and J. de Vries. 1998. Interleukin-13. In The Cytokine Handbook. A. Thompson, Editor. Academic Press, p. 427 – 442.
Kitamura, T., et al. 1989. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334.