Contents: Recombinant Human IL-12 p70 Single-Use ELISA RSG Standard
Catalog Number: 39-8129
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: Insect cells infected with baculovirus: human p40, amino acids met 1-ser 328, (accession # NM_002187) was co-expressed with human p35, amino acids met 1-ser-253, (accession # NM_000882).
Purity: Greater than 98%, as determined by SDS-PAGE
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Description
Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-γ) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-γ and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human IL-12 p70 is biologically active. The intended use of the Recombinant Human IL-12 p70 Single-Use ELISA RSG Standard is as a standard for human IL-12 p70 Ready-SET-Go! ELISA and human IL-12 p70 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (2000 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the human IL-12 p70 ELISA pair (B-T21/C8.6).
References
Isler, P., et al. 1999. Am. J. Respir. Cell Mol. Biol. 20: 270-278.
