Product Information

Contents: Recombinant Mouse IL-12 p70 Single-Use ELISA RSG Standard
Catalog Number: 39-8121
Sizes: 4 pack
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 335, (accession # NM_008352) was co-expressed with mouse p35, amino acids met 1-ala 215, (accession # NM_008351).
Purity: Greater than 98%, as determined by SDS-PAGE

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8121	Recombinant Mouse IL-12 (Interleukin-12, IL12) p70	N/A	N/A	BA ELISA std
34-8121	Carrier-Free Recombinant Mouse IL-12 (Interleukin-12, IL12) p70	N/A	N/A	BA ELISA
39-8121	Single-Use ELISA RSG Standard Recombinant Mouse IL-12 (Interleukin-12, IL12) p70	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

eBioscience’s recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-12 p70 is biologically active. The intended use of the Recombinant Mouse IL-12 p70 Single-Use ELISA RSG Standard is as a standard for mouse IL-12 p70 Ready-SET-Go! ELISA and mouse IL-12 p70 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (2000 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (2000 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-12 p70 ELISA pair (C18.2/C17.8).

References

Isler, P., et al. 1999. Am. J. Respir. Cell Mol. Biol. 20: 270-278.